FoCuS-point

FoCuS-point is a user-friendly and efficient stand-alone software designed to process fluorescence correlation spectroscopy (FCS) data obtained from super-resolution STED microscopy experiments. The software utilizes advanced time-correlated single-photon counting (TCSPC) correlation algorithms along with time-gated filtering and innovative data visualization to handle batches of data. FoCuS-point also includes advanced fitting algorithms which allow the parameters of the correlation curves and the kinetics of diffusion to be established quickly and efficiently.

Topic

Data visualisation;Physics

Detail

  • Operation: Filtering;Correlation;Visualisation

  • Software interface: Command-line user interface

  • Language: Python

  • License: GNU General Public License v2.0

  • Cost: Free

  • Version name: 1.16.208

  • Credit: Alfred Benzon Foundation, Medical Research Council, MRC-BBSRC-EPSRC, Wolfson Foundation and Wellcome Trust.

  • Input: '.SIN' and '.fcs' correlated files and '.csv' files, .pt3 and .ptu files created using PicoQuant

  • Output: -

  • Contact: dominic.waithe@imm.ox.ac.uk

  • Collection: -

  • Maturity: Stable

Publications

  • FoCuS-point: software for STED fluorescence correlation and time-gated single photon counting.
  • Waithe D, et al. FoCuS-point: software for STED fluorescence correlation and time-gated single photon counting. FoCuS-point: software for STED fluorescence correlation and time-gated single photon counting. 2016; 32:958-60. doi: 10.1093/bioinformatics/btv687
  • https://doi.org/10.1093/bioinformatics/btv687
  • PMID: 26589275
  • PMC: PMC5939892

Download and documentation

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