Hierarchicell

Hierarchicell is an R-package for power calculations in single-cell RNA-seq experiments, addressing the overestimation issue inherent in existing calculators by focusing on the number of independent experimental units rather than total cell count. It simulates hierarchical correlation structures within samples to provide accurate power estimates for differential expression tests. This tool models various data aspects, including gene dropout rates and intra- and inter-individual variation. Hierarchicell supports both binary and continuous phenotype analyses, catering to user-defined experimental unit numbers and cell counts within those units. Available on GitHub, it enhances experimental design accuracy in single-cell RNA-seq studies.