InSpecT

InsPecT is a powerful software tool designed to identify post-translational modifications in proteins using tandem mass spectrometry data. It achieves this task by constructing database filters that are highly effective at identifying relevant peptides within large databases. These filters use peptide sequence tags to efficiently reduce the size of the database while maintaining a high level of accuracy. Additionally, InsPecT employs novel algorithms for scoring and validating modifications, allowing it to accurately identify modified peptides even when there are numerous possible variations. Compared to other database search tools, InsPecT is significantly faster and more accurate, making it a valuable resource for researchers studying protein modification patterns.

Topic

Small molecules;Proteomics;Proteomics experiment;Protein modifications

Detail

  • Operation: PTM identification;Validation;Tag-based peptide identification;de Novo sequencing;Peptide database search

  • Software interface: Command-line interface

  • Language: Python

  • License: -

  • Cost: -

  • Version name: -

  • Credit: -

  • Input: Mass spectrometry spectra [mzXML] [MGF] [pkl] [dta] [mzData], Sequence set (protein) [FASTA]

  • Output: Peptide identification [Textual format]

  • Contact: Natalie Castellana ncastell@cs.ucsd.edu

  • Collection: -

  • Maturity: -

Publications

  • InsPecT: identification of posttranslationally modified peptides from tandem mass spectra.
  • Tanner S, et al. InsPecT: identification of posttranslationally modified peptides from tandem mass spectra. InsPecT: identification of posttranslationally modified peptides from tandem mass spectra. 2005; 77:4626-39. doi: 10.1021/ac050102d
  • https://doi.org/10.1021/ac050102d
  • PMID: 16013882
  • PMC: -

Download and documentation

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