MoNaS

MoNaS (Mosquito Na+ channel mutation Search) is a pipeline designed to automate the process of identifying amino acid substitutions in the voltage-gated sodium channel (VGSC) protein from next-generation sequencing (NGS) reads. The tool is specifically developed to facilitate the discovery of novel amino acid variants that confer insecticide resistance in the VGSC of insect pests, particularly mosquitoes.

Key features of MoNaS:

1. Automated analysis: MoNaS streamlines the bioinformatic process by automatically calling amino acid substitutions in the VGSC from NGS reads.

2. Comparison with known resistance mutations: The pipeline compares the identified amino acid substitutions with a database of known resistance mutations, helping researchers quickly identify potential novel resistance variants.

3. Designed for insect pests: MoNaS is specifically tailored for the analysis of VGSC in clinically and agriculturally important insect pests, such as mosquitoes.

4. Facilitates population genetic studies: By identifying resistance alleles, MoNaS can aid in population genetic studies investigating the origin, selection, and migration of insecticide resistance in insect populations.