MrNV

MrNV analyzes transcriptomes of Macrobrachium rosenbergii post-larvae to characterize molecular responses to infection by Macrobrachium rosenbergii nodavirus (MrNV).

Key Features:

• Transcriptome Assembly: Assembles RNA sequencing (RNAseq) data into 96,362 unigenes with an N50 of 1308 bp and an ExN50 of 94%, reporting BUSCO completeness of 83.4%, fragmentation 13%, missing 3.3%, and duplication 16.2%.
• Functional Annotation: Annotates the assembled transcriptome against NCBI non-redundant arthropod (33.75%), UniProt (26.73%), Gene Ontology (GO) (18.98%), EggNOG (20.88%), and KEGG pathways (20.46%).
• Differential Abundance Analysis: Performs differential abundance analysis using EdgeR, identifying 2,413 significantly up-regulated and 3,125 significantly down-regulated genes in response to MrNV infection and highlighting transcripts such as antiviral proteins, C-type lectins, prophenol oxidase, caspases, ADP ribosylation factors, and dicer.
• Validation: Validates differential expression results by quantitative PCR (qPCR) to confirm candidate immune-related transcripts associated with MrNV infection.

Scientific Applications:

• Immune response characterization: Characterizes transcriptional changes underlying innate immune responses in M. rosenbergii post-larvae to MrNV infection.
• Host-pathogen interaction analysis: Identifies antiviral and immune-related genes and pathways involved in responses to Macrobrachium rosenbergii nodavirus (MrNV).
• Disease mitigation research: Provides transcriptomic evidence to inform strategies for understanding and mitigating White Tail Disease impacts in aquaculture.

Methodology:

RNA sequencing (RNAseq) followed by transcriptome assembly, functional annotation against NCBI non-redundant arthropod, UniProt, GO, EggNOG, and KEGG databases, and differential abundance analysis using EdgeR.