NanoMethViz
"NanoMethViz" is an R package designed to facilitate the effective visualization of DNA methylation patterns derived from long-read nanopore sequencing technology. The tool accommodates methylation calls from various methylation callers and efficiently manages large datasets using a compressed format. NanoMethViz offers diverse visualization options, allowing users to explore methylation changes at different resolutions. It includes sample-level visualizations using multidimensional scaling, aggregated methylation profiles for features like genes or CpG islands, and high-resolution views of methylation patterns across individual reads using a spaghetti plot. The tool enhances the convenience of handling methylation signals and seamlessly integrates with existing methylation analysis tools available in the Bioconductor project.
Topic
Epigenetics;Methylated DNA immunoprecipitation;DNA;Gene transcripts;Gene expression
Detail
Operation: Methylation calling;Visualisation;Aggregation;Whole genome methylation analysis;PTM site prediction
Software interface: Library
Language: R
License: Apache License, Version 2.0
Cost: Free
Version name: 2.8.1
Credit: Australian National Health and Medical Research Council (NHMRC), a Bellberry-Viertel, Senior Medical Research Fellowship.
Input: -
Output: -
Contact: Shian Su su.s@wehi.edu.au
Collection: -
Maturity: Stable
Publications
- NanoMethViz: an R/Bioconductor package for visualizing long-read methylation data
- Su S, Gouil Q, Blewitt ME, Cook D, Hickey PF, Ritchie ME. NanoMethViz: An R/Bioconductor package for visualizing long-read methylation data. PLoS Comput Biol. 2021 Oct 25;17(10):e1009524. doi: 10.1371/journal.pcbi.1009524. PMID: 34695109; PMCID: PMC8568149.
- https://doi.org/10.1371/journal.pcbi.1009524
- PMID: 34695109
- PMC: PMC8568149
Download and documentation
Source: https://bioconductor.org/src/contrib/NanoMethViz_2.8.1.tar.gz
Documentation: https://bioconductor.org/manuals/NanoMethViz/man/NanoMethViz.pdf
Links: https://bioconductor.org/vignettes/NanoMethViz/inst/doc/UsersGuide.html
Links: https://bioconductor.org/vignettes/NanoMethViz/inst/doc/UsersGuide.R
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