NanoOK

NanoOK is a comprehensive tool for real-time, long-read sequencing quality control and error profile analysis from the Oxford Nanopore MinION sequencer. NanoOK provides detailed tabular and graphical output, plus an in-depth multi-page PDF report, for metagenomic and multiplexed samples, and works with four popular Nanopore aligners, with easy extensibility to include others.

Topic

Sequence analysis;Genomics;Nucleic acids

Detail

  • Operation: Sequence analysis;Sequence alignment

  • Software interface: Command-line user interface

  • Language: Java

  • License: GNU General Public License v3

  • Cost: Free

  • Version name: 1.33

  • Credit: BBSRC National Capability Grant.

  • Input: -

  • Output: -

  • Contact: richard.leggett@earlham.ac.uk

  • Collection: -

  • Maturity: -

Publications

  • Rapid MinION profiling of preterm microbiota and antimicrobial-resistant pathogens
  • Leggett, R.M., Alcon-Giner, C., Heavens, D. et al. Rapid MinION profiling of preterm microbiota and antimicrobial-resistant pathogens. Nat Microbiol 5, 430–442 (2020). https://doi.org/10.1038/s41564-019-0626-z
  • https://doi.org/10.1038/s41564-019-0626-z
  • PMID: 31844297
  • PMC: PMC7044117
  • NanoOK: multi-reference alignment analysis of nanopore sequencing data, quality and error profiles.
  • Leggett RM, et al. NanoOK: multi-reference alignment analysis of nanopore sequencing data, quality and error profiles. NanoOK: multi-reference alignment analysis of nanopore sequencing data, quality and error profiles. 2016; 32:142-4. doi: 10.1093/bioinformatics/btv540
  • https://doi.org/10.1093/bioinformatics/btv540
  • PMID: 26382197
  • PMC: PMC4681994

Download and documentation

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