PSP-GNM

'PSP-GNM' is a novel software tool designed to address the critical biological problem of understanding the impact of missense mutations on protein stability. Missense mutations in the genome can alter one or more amino acids in a protein, potentially leading to changes in the stability of the encoded proteins.
PSP-GNM employs a unique approach called the Protein Stability Prediction with a Gaussian Network Model (PSP-GNM) to assess the unfolding Gibbs free energy change (ΔΔG) and evaluate the effects of single amino acid substitutions on protein stability. Specifically, PSP-GNM utilizes a coarse-grained Gaussian Network Model (GNM) that considers interactions between amino acids weighted by the Miyazawa-Jernigan statistical potential. The tool simulates partial unfolding of both the wildtype and mutant protein structures, and then calculates ΔΔG by measuring the difference in energies and entropies of the unfolded wildtype and mutant proteins.