Primer3_masker

'Primer3_masker' is a software tool that uses statistical modeling and k-mer based masking to detect and mask unsuitable regions for PCR amplification in eukaryotic genomes. It is available as a standalone version for Linux and macOS on GitHub and has been integrated into Primer3. The tool is designed to address the problem of non-specific binding sites, which can cause PCR primer failure rates to be high when amplifying regions from large eukaryotic genomes. The tool helps to ensure the specificity of primer binding sites and makes the primer design process more efficient.