RNF
The software tool RNF (Read Naming Format) aims to solve the problem of evaluating and comparing read mappers using simulated reads, which often requires explicit compatibility between the simulator and the evaluation tool. RNF introduces a generic format for encoding information about original positions of simulated reads called Read Naming Format (Rnf), and an associated software package called RnfTools that includes two components: MIShmash, which transforms generated reads from popular simulating tools into Rnf format, and LAVEnder, which evaluates a read mapper using simulated reads in Rnf format.
Topic
DNA;Sequencing;Nucleic acid structure analysis
Detail
Operation: Read pre-processing
Software interface: Command-line user interface
Language: Python
License: MIT License
Cost: Free
Version name: 0.3.1.3
Credit: ABS4NGS grant, Labex Bézout of the French government (program Investissement d’Avenir).
Input: -
Output: -
Contact: karel.brinda@univ-mlv.fr;valentina.boeva@curie.fr;gregory.kucherov@univ-mlv.fr
Collection: -
Maturity: -
Publications
- RNF: a general framework to evaluate NGS read mappers.
- Břinda K, et al. RNF: a general framework to evaluate NGS read mappers. RNF: a general framework to evaluate NGS read mappers. 2016; 32:136-9. doi: 10.1093/bioinformatics/btv524
- https://doi.org/10.1093/bioinformatics/btv524
- PMID: 26353839
- PMC: PMC4681991
Download and documentation
Source: https://github.com/karel-brinda/rnftools/releases/tag/0.3.1.3
Documentation: https://rnftools.readthedocs.io/en/latest/
Home page: http://karel-brinda.github.io/rnftools/
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