ReVac

ReVac is a software tool that accelerates the discovery of potential vaccine candidates (PVCs) from multiple bacterial proteomes. It employs a comprehensive approach that combines feature prediction programs and a scoring system based on curated datasets of experimentally validated antigens and non-protective antigens. ReVac assesses protein conservation across several genomes and identifies core and dispensable genome components, which is crucial for determining the degree of conservation of PVCs among the population of isolates for a given pathogen. The tool also considers DNA and protein repeats that may result in variable expression of PVCs. ReVac ranks PVCs based on conservation and overall feature-based scoring, prioritizing them for subsequent experimental testing. By performing multi-genome analyses, ReVac provides a comprehensive overview of PVCs from a pan-genome perspective, essential for bacterial subunit vaccine design.