RiboProfiling

RiboProfiling is an open-source package to analyze ribosome profiling (Ribo-seq) data, which involves the selective sequencing of translated RNA regions. The package provides a comprehensive pipeline for processing and analyzing ribosome footprint data, encompassing all key steps in a single R workflow.

Key Features of RiboProfiling:

Input Handling: Accepts alignment (BAM) files as input, streamlining the data processing workflow.

Footprint Quantification: Quantifies ribosome footprints at the transcript level, enabling detailed translation analysis.

Motif Identification: This method detects the accumulation of ribosome footprints on specific amino acids or multi-acid motifs, helping to identify regions of ribosome pausing or differential translation.

Automated Reporting: Generates summary graphs and data quantification automatically, facilitating straightforward interpretation of results.

Quality Assessment: Provides tools for assessing the quality of Ribo-seq experiments, ensuring reliable and reproducible results.