SplicingFactory

The software tool 'SplicingFactory' is an R package to characterize and analyze RNA isoform diversity using isoform-level expression measurements. It addresses the stochastic nature of the splicing process, allowing researchers to investigate changes in RNA isoform diversity between conditions or samples. The package calculates various RNA isoform diversity metrics and facilitates their comparison across conditions. Through testing and analysis, it explores factors such as RNA-seq quantification tools, quantification uncertainty, gene expression levels, and isoform numbers that may influence isoform diversity calculations.