TomahaqCompanion

TomahaqCompanion is a software tool that facilitates and optimizes the Triggered by Offset, Multiplexed, Accurate mass, High Resolution, and Absolute Quantitation (TOMAHAQ) method in targeted proteomics. TOMAHAQ is a sophisticated approach that enhances the capabilities of targeted proteomics by enabling sensitive and accurate multiplexed quantification of peptides. This method integrates peptide and sample multiplexing with a complex data collection scheme involving multiple mass spectrometry (MS) analysis stages. The technique's strength lies in combining high-resolution mass accuracy with absolute quantitation, making it particularly valuable for precise and comprehensive proteomic studies.

Given the complexity and precision of the TOMAHAQ method, TomahaqCompanion serves as an essential utility for researchers engaged in targeted proteomics experiments. The software streamlines the setup and execution of TOMAHAQ assays, addressing the challenges associated with the method's intricate data collection and analysis requirements. By simplifying the process of designing and implementing TOMAHAQ experiments, TomahaqCompanion enables researchers to fully leverage the method's advantages for sensitive and multiplexed quantification.

Topic

Proteomics

Detail

  • Operation: Design

  • Software interface: Desktop application

  • Language: C#

  • License: The GNU General Public License v3.0

  • Cost: Free with restrictions

  • Version name: 1.0.2

  • Credit: NIH.

  • Input: Protein sequence (raw) [CSV], Mass spectrometry spectra [Thermo RAW]

  • Output: Data

  • Contact: Christopher M. Rose rose.christopher@gene.com

  • Collection: -

  • Maturity: Stable

Publications

  • TomahaqCompanion: A Tool for the Creation and Analysis of Isobaric Label Based Multiplexed Targeted Assays.
  • Rose CM, et al. TomahaqCompanion: A Tool for the Creation and Analysis of Isobaric Label Based Multiplexed Targeted Assays. TomahaqCompanion: A Tool for the Creation and Analysis of Isobaric Label Based Multiplexed Targeted Assays. 2019; 18:594-605. doi: 10.1021/acs.jproteome.8b00767
  • https://doi.org/10.1021/acs.jproteome.8b00767
  • PMID: 30501201
  • PMC: PMC7315617

Download and documentation


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