XPRESS

XPRESS is a powerful software tool that enables the systematic identification and quantification of proteins in cells' microsomal fraction. This comprehensive approach consists of three main steps: (1) the preparation of microsomal fractions from cells or tissues representing different states; (2) the covalent tagging of the proteins with isotope-coded affinity tag (ICAT) reagents followed by proteolysis of the combined labeled protein samples; and (3) the isolation, identification, and quantification of the tagged peptides by multidimensional chromatography, automated tandem mass spectrometry, and computational analysis of the obtained data.

This cutting-edge method was successfully used to identify and determine the ratios of the abundance of each of the 491 proteins contained in the microsomal fractions of naïve and in vitro-differentiated human myeloid leukemia (HL-60) cells. The XPRESS tool and the new software tools that support it are ideal for the large-scale, quantitative analysis of membrane proteins and other classes of proteins refractory to standard proteomics technology.

Topic

Proteomics;Proteomics experiment

Detail

  • Operation: Protein quantification;Labeled quantification;Isotope-coded protein label

  • Software interface: Command-line user interface;Graphical user interface

  • Language: C++

  • License: GNU General Public License, version 2

  • Cost: Free

  • Version name: -

  • Credit: The University of Washington Royalty Research Fund, the Merck Genome Research Institute, the National Cancer Institute, the National Institutes of Health.

  • Input: -

  • Output: -

  • Contact: aebersold@systemsbiology.org

  • Collection: -

  • Maturity: -

Publications

  • Quantitative profiling of differentiation-induced microsomal proteins using isotope-coded affinity tags and mass spectrometry.
  • Han DK, et al. Quantitative profiling of differentiation-induced microsomal proteins using isotope-coded affinity tags and mass spectrometry. Quantitative profiling of differentiation-induced microsomal proteins using isotope-coded affinity tags and mass spectrometry. 2001; 19:946-51. doi: 10.1038/nbt1001-946
  • https://doi.org/10.1038/nbt1001-946
  • PMID: 11581660
  • PMC: PMC1444949

Download and documentation

< Back to DB search