crisprVerse

crisprVerse IS an open-source ecosystem of R packages that enables efficient gRNA design and annotation for any RNA- or DNA-targeting nucleases, such as Cas9, Cas12, and Cas13. This includes CRISPR knockout (CRISPRko), CRISPR activation (CRISPRa), CRISPR interference (CRISPRi), CRISPR base editing (CRISPRbe), and CRISPR knockdown (CRISPRkd). The core package, crisprDesign, offers users a unified interface to add off-target annotations, rich gene and SNP annotations, and on- and off-target activity scores.

Topic

Gene transcripts;DNA polymorphism;Sequencing;Workflows;Transcription factors and regulatory sites

Detail

  • Operation: Editing;SNP annotation;Genome indexing

  • Software interface: Library

  • Language: R, C++, C, TeX, Python

  • License: The MIT License

  • Cost: Free

  • Version name: -

  • Credit: -

  • Input: -

  • Output: -

  • Contact: Jean-Philippe Fortin fortin946@gmail.com

  • Collection: -

  • Maturity: -

Publications

  • A comprehensive Bioconductor ecosystem for the design of CRISPR guide RNAs across nucleases and technologies.
  • Hoberecht L, et al. A comprehensive Bioconductor ecosystem for the design of CRISPR guide RNAs across nucleases and technologies. A comprehensive Bioconductor ecosystem for the design of CRISPR guide RNAs across nucleases and technologies. 2022; 13:6568. doi: 10.1038/s41467-022-34320-7
  • https://doi.org/10.1038/S41467-022-34320-7
  • PMID: 36323688
  • PMC: PMC9630310

Download and documentation

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