flowAI

The software tool 'flowAI' is an R package that offers two methods to clean flow cytometry (FCM) files from unwanted events. It includes an automatic method and an interactive method with a graphical user interface. The software checks and removes suspected anomalies based on abrupt changes in flow rate, instability of signal acquisition, and outliers in the dynamic range. The software generates a summary of the quality assessment for each file analyzed, seeking to improve the results of both manual and automatic FCM data analysis.

Topic

Data quality management

Detail

  • Operation: Spectral analysis

  • Software interface: Command-line user interface;Library

  • Language: R

  • License: GNU General Public License, version 2

  • Cost: Free

  • Version name: 1.28.0

  • Credit: -

  • Input: -

  • Output: -

  • Contact: gianni@liverpool.ac.uk

  • Collection: -

  • Maturity: Stable

Publications

  • flowAI: automatic and interactive anomaly discerning tools for flow cytometry data.
  • Monaco G, et al. flowAI: automatic and interactive anomaly discerning tools for flow cytometry data. flowAI: automatic and interactive anomaly discerning tools for flow cytometry data. 2016; 32:2473-80. doi: 10.1093/bioinformatics/btw191
  • https://doi.org/10.1093/bioinformatics/btw191
  • PMID: 27153628
  • PMC: -

Download and documentation

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