flowDensity

The software tool 'flowDensity' is an algorithm that automates a predefined manual gating approach for reproducible, high-throughput analysis of flow cytometry data. The algorithm uses a sequential bivariate gating approach to generate a set of predefined cell populations and selects the best cut-off for individual markers using characteristics of the density distribution.

Topic

Cell biology

Detail

  • Operation: Spectral analysis

  • Software interface: Command-line user interface;Library

  • Language: R

  • License: Artistic-2.0

  • Cost: Free

  • Version name: 1.32.0

  • Credit: National Institutes of Health, the Human Immunology Consortium, National Science and Engineering Research Council.

  • Input: -

  • Output: -

  • Contact: Mehrnoush Malek mmalekes@bccrc.ca

  • Collection: -

  • Maturity: Stable

Publications

  • flowDensity: reproducing manual gating of flow cytometry data by automated density-based cell population identification.
  • Malek M, et al. flowDensity: reproducing manual gating of flow cytometry data by automated density-based cell population identification. flowDensity: reproducing manual gating of flow cytometry data by automated density-based cell population identification. 2015; 31:606-7. doi: 10.1093/bioinformatics/btu677
  • https://doi.org/10.1093/bioinformatics/btu677
  • PMID: 25378466
  • PMC: PMC4325545

Download and documentation

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