flowFit

The software tool 'flowFit' is a Bioconductor package that performs quantitative analysis of cell proliferation in tracking dye-based experiments. The algorithm uses a mathematical model to deconvolute the collected data into a set of peaks, whereby each peak corresponds to a subpopulation of cells that have divided N times. The package is distributed as an R Bioconductor library and is validated by retrospective analysis of published proliferation-tracking experiments. The algorithm might be applied to numerous areas of cell biology in which quantitative deconvolution of tracking dye-based experiments is desired, including stem cell research.

Topic

Laboratory techniques;Data management;Biophysics

Detail

  • Operation: Spectral analysis;Visualisation;Data handling

  • Software interface: Command-line user interface;Library

  • Language: R

  • License: Artistic-2.0

  • Cost: Free

  • Version name: 1.6.0

  • Credit: The Associazione Italiana per la Ricerca sul Cancro (AIRC), the Italian Ministries of Education-University-Research (MIUR) and of Health, the Monzino Foundation and the European Research Council, the CARIPLO Foundation, G. Vollaro Foundation

  • Input: -

  • Output: -

  • Contact: Davide Rambaldi davide.rambaldi@gmail.com

  • Collection: -

  • Maturity: Mature

Publications

  • flowFit: a Bioconductor package to estimate proliferation in cell-tracking dye studies.
  • Rambaldi D, et al. flowFit: a Bioconductor package to estimate proliferation in cell-tracking dye studies. flowFit: a Bioconductor package to estimate proliferation in cell-tracking dye studies. 2014; 30:2060-5. doi: 10.1093/bioinformatics/btu127
  • https://doi.org/10.1093/bioinformatics/btu127
  • PMID: 24681909
  • PMC: -

Download and documentation

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