m7G-MaP-seq

m7G-MaP-seq is a high-throughput sequencing method that detects N7-methylguanosine (m7G) modifications in RNA at single-nucleotide resolution. The method involves converting m7G-modified positions to abasic sites through reduction with sodium borohydride, which is then directly recorded as mutations in the cDNA during reverse transcription. By comparing the mutation rates between reduced and control samples and using replicates for statistical significance calculations, m7G-MaP-seq can efficiently identify known and novel m7G modifications in various RNA species, such as rRNA and tRNA, across different organisms.

Topic

Functional, regulatory and non-coding RNA;Gene transcripts;RNA-Seq

Detail

  • Operation: Post-translation modification site prediction;miRNA target prediction;Expression analysis

  • Software interface: Library

  • Language: R,Shell

  • License: Not stated

  • Cost: Free of charge

  • Version name: -

  • Credit: The Novo Nordisk Foundation and the Danish Council for Strategic Research.

  • Input: -

  • Output: -

  • Contact: Jeppe Vinther jvinther@bio.ku.dk

  • Collection: -

  • Maturity: -

Publications

  • Detection of internal N7-methylguanosine (m7G) RNA modifications by mutational profiling sequencing.
  • Enroth C, et al. Detection of internal N7-methylguanosine (m7G) RNA modifications by mutational profiling sequencing. Detection of internal N7-methylguanosine (m7G) RNA modifications by mutational profiling sequencing. 2019; 47:e126. doi: 10.1093/nar/gkz736
  • https://doi.org/10.1093/NAR/GKZ736
  • PMID: 31504776
  • PMC: PMC6847341

Download and documentation

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