pRESTO

pRESTO is a computational pipeline developed to process high-throughput sequencing reads from lymphocyte receptor studies. It produces error-corrected, sorted, and annotated sequence sets and provides metrics at each step. pRESTO supports multiplexed primer pools, single- or paired-end reads, and single-molecule identifier technologies. The software can efficiently process millions of sequences, and it has been tested on data generated by Roche and Illumina platforms. pRESTO is freely available for academic use.

Topic

Sequencing;DNA;Immunology

Detail

  • Operation: Nucleic acid sequence analysis

  • Software interface: Command-line user interface

  • Language: Python

  • License: GNU Affero General Public License v3

  • Cost: Free

  • Version name: 0.7.1

  • Credit: National Library of Medicine, United States-Israel Binational Science Foundation, National Institutes of Health, EMD-Merck-Serono sponsored Grant for Multiple Sclerosis Research Innovation; Race to Erase MS.

  • Input: -

  • Output: -

  • Contact: Steven H. Kleinstein steven.kleinstein@yale.edu

  • Collection: -

  • Maturity: -

Publications

  • pRESTO: a toolkit for processing high-throughput sequencing raw reads of lymphocyte receptor repertoires.
  • Vander Heiden JA, et al. pRESTO: a toolkit for processing high-throughput sequencing raw reads of lymphocyte receptor repertoires. pRESTO: a toolkit for processing high-throughput sequencing raw reads of lymphocyte receptor repertoires. 2014; 30:1930-2. doi: 10.1093/bioinformatics/btu138
  • https://doi.org/10.1093/bioinformatics/btu138
  • PMID: 24618469
  • PMC: PMC4071206

Download and documentation

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