pamgeneAnalyzeR

pamgeneAnalyzeR is an R package to facilitate the high-throughput profiling of kinase activity, an experimental approach crucial for studying the dynamics of protein phosphorylation. This post-translational modification, predominantly catalyzed by protein kinases, significantly enriches the functional diversity of the proteome and has profound implications on various physiological processes and disease states.

Utilizing technologies like PamChip arrays from PamGene, which are dedicated to measuring kinase activity, pamgeneAnalyzeR offers an efficient alternative to manual data extraction from peptide microarray images. This tool not only automates the data extraction process but also ensures that the data is processed in a reproducible and robust manner, ready for downstream analysis.

Topic

Protein modifications;Proteomics;Workflows;Microarray experiment;Small molecules

Detail

  • Operation: Standardisation and normalisation;PTM site prediction;Quantification

  • Software interface: Command-line interface

  • Language: R

  • License: GNU General Public License, version 3

  • Cost: -

  • Version name: 0.1.0

  • Credit: -

  • Input: -

  • Output: -

  • Contact: Amel Bekkar amelbekkar@gmail.com

  • Collection: -

  • Maturity: -

Publications

  • PamgeneAnalyzeR: open and reproducible pipeline for kinase profiling.
  • Bekkar A, et al. PamgeneAnalyzeR: open and reproducible pipeline for kinase profiling. PamgeneAnalyzeR: open and reproducible pipeline for kinase profiling. 2020; 36:5117-5119. doi: 10.1093/bioinformatics/btz858
  • https://doi.org/10.1093/BIOINFORMATICS/BTZ858
  • PMID: 31922550
  • PMC: PMC7755406

Download and documentation


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