prokka

Prokka is a software tool that significantly simplifies the process of fully annotating a draft bacterial genome. With the advent of multiplex capability and the high yield of DNA sequencing instruments, bacterial whole genome sequencing has become a routine affair. However, annotating all relevant genomic features on the contigs can be time-consuming. Existing web- and email-based systems are not applicable for sensitive data or integrating into computational pipelines.

Prokka solves this problem by providing a command-line tool to fully annotate a draft bacterial genome in about 10 minutes using a typical desktop computer. The software produces standards-compliant output files that genome browsers can further analyze or view.