qPCRtools

'qPCRtools' is an R package designed for the analysis of gene amplification efficiency in biological research, particularly for the quantification of gene expression levels using the quantitative polymerase chain reaction (qPCR) technique. Gene amplification efficiency calculation is a crucial step in processing qPCR data, as it helps researchers decide which method to use for computing gene expression levels.
This software offers several key functionalities:
1. Analysis of Gene Amplification Efficiency: 'qPCRtools' enables users to assess the efficiency of gene amplification, a critical aspect of qPCR data analysis.
2. Gene Expression Quantification: It provides three approaches for determining gene expression levels, including: - The conventional curve-based method.
- The 2^(-ΔΔCt) method, commonly used for relative quantification.
- The SATQPCR method, which can be used for more accurate quantification.

3. Statistical Data and Visualization: The package generates a table containing statistical data for each of the above methods and offers the option to create graphical representations of the results, such as box or bar plots.