scRNAss

The software tool 'scRNAss' is designed to address the challenge of full-length transcript reconstruction in single-cell RNA-seq data analysis caused by dropout events. It applies explicit strategies to impute lost information and a combining strategy to infer transcripts using scRNA-seq. The tool was evaluated on simulated and biological datasets and demonstrated superior performance compared to existing RNA-seq assemblers. It also showed a remarkable capability of recovering unknown 'novel' isoforms and high computational efficiency.