seqbias

"seqbias" is a software tool designed to tackle the challenge of sequence bias in RNA-Seq experiments, a factor that can significantly impact the accuracy of sequence abundance quantification. This bias can stem from protocol-specific sources, such as polymerase chain reaction (PCR) amplification or differences in primer affinities, leading to complications in tasks like de novo gene annotation and transcript quantification.

Seqbias measures and corrects these biases through a simple graphical model that operates independently of existing gene annotations. This feature ensures its applicability to a wide range of datasets with minimal assumptions required. Moreover, the model selection process within seqbias is automated, further enhancing its usability.