A tool to design target-specific primers for polymerase chain reaction. Te tool achieves its sensitivity by a combination of BLAST and a global alignment algorithm to get a full primer-target alignment.
Biology, Genetics, Molecular genetics, Genetic variation, DNA polymorphism
Ye J, Coulouris G, Zaretskaya I, Cutcutache I, Rozen S, Madden TL "Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction." BMC Bioinformatics 2012 https://doi.org/10.1186/1471-2105-13-134
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