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Primer-BLAST

Primer-BLAST

A tool to design target-specific primers for polymerase chain reaction. Te tool achieves its sensitivity by a combination of BLAST and a global alignment algorithm to get a full primer-target alignment.

Topic

Biology, Genetics, Molecular genetics, Genetic variation, DNA polymorphism

Details

  • Operation: Primer and probe design
  • Software interface: Web user interface
  • Language: -
  • License: Not stated
  • Cost: Free
  • Version name: -
  • Credit: Ye J, Coulouris G, Zaretskaya I, Cutcutache I, Rozen S, Madden TL
  • Contact: -
  • Collection: -

Publications

Ye J, Coulouris G, Zaretskaya I, Cutcutache I, Rozen S, Madden TL "Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction." BMC Bioinformatics 2012 https://doi.org/10.1186/1471-2105-13-134
PMID: 22708584
PMCID: PMC3412702


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