A tool for quality control of RNA-seq data. The RNA-SeQC package has functions for computing various quality metrics, such as alignment quality, duplication rates, GC bias, rRNA content, coverage continuity, covered alignment regions, transcript count, and 3'/5' bias. It produces Read counts, coverage, correlation quality control metrics, and is also suitable for use with scRNA-seq data sets.
RNA-seq; scRNA-seq
DeLuca DS, Levin JZ, Sivachenko A, Fennell T, Nazaire MD, Williams C, Reich M, Winckler W, Getz G "RNA-SeQC: RNA-seq metrics for quality control and process optimization." Bioinformatics. 2012 Jun 1;28(11):1530-2. Epub 2012 Apr 25. https://doi.org/10.1093/bioinformatics/bts196
PMID: 22539670
PMCID: PMC3356847
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