RSeQC tool consists of a set of tools for quality assessment of RNA-seq data. The RSeQC package includes functions for sequence quality, nucleotide composition, GC, and PCR bias, sequencing depth, strand specificity, mapped reads distribution, coverage uniformity, RNA integrity, and genomic read distribution.
Sequencing
Wang L, Wang S, Li W "RSeQC: quality control of RNA-seq experiments." Bioinformatics. 2012 Aug 15;28(16):2184-5. Epub 2012 Jun 27. https://doi.org/10.1093/bioinformatics/bts356
PMID: 22743226
Wang L, Nie J, Sicotte H, Li Y, Eckel-Passow JE, Dasari S, Vedell PT, Barman P, Wang L, Weinshiboum R, Jen J, Huang H, Kohli M, Kocher JP "Measure transcript integrity using RNA-seq data" BMC Bioinformatics. 2016 Feb 3;17:58. https://doi.org/10.1186/s12859-016-0922-z
PMID: 26842848
PMCID: PMC4739097
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