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Rnnotator

Rnnotator

Rnnotator is a pipeline tool for the generation of full-length transcript models by computing de novo assemblies of RNA-seq data sets. The Rnnotator algorithm specifically addresses issues arising from poor read quality and, read length, and can make deep coverage assemblies. It can use paired-end, stranded reads, multiple insert sizes, and works on multiple CPUs. Obtaining Rnnotator requires a license unless you are collaborating with the developer. Contact Lawrence Berkeley National Laboratory David Gilbert at DEGilbert_at_lbl.gov for more information.

Topic

Sequence assembly; RNA-Seq; Sequencing

Details

  • Operation: De-novo assembly
  • Input: FASTQ
  • Output: -
  • Software interface: Command-line user interface
  • Language: Perl
  • Operating system: Linux
  • License: Not stated
  • Cost: Free for academic collaborators
  • Version name: -
  • Credit: The Office of Science of the U.S. Department of Energy, the National Institute of Allergy and Infectious Diseases (NIAID), the National Institutes of Health (NIH).
  • Contact: David Gilbert DEGilbert _at_ lbl.gov | Jeffrey Martin JAMartin _at_ lbl.gov | Zhong Wang ZhongWang _at_ lbl.gov | Xiandong Meng XiandongMeng _at_ lbl.gov
  • Collection: -

Publications

Martin J, Bruno VM, Fang Z, Meng X, Blow M, Zhang T, Sherlock G, Snyder M, Wang Z "Rnnotator: an automated de novo transcriptome assembly pipeline from stranded RNA-Seq reads." BMC Genomics. 2010 Nov 24;11:663. https://doi.org/10.1186/1471-2164-11-663
PMID: 21106091
PMCID: PMC3152782


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