TIGAR2 is a tool to quantify transcript isoforms in RNA-seq data sets. The TIGAR2 algorithm applies a variational Bayesian inference, and it can also model sequencing errors. It can use Bowtie2 and BWA-MEM tools in the computational pipeline.
Genetics; RNA-Seq; RNA splicing
Nariai N, Kojima K, Mimori T, Sato Y, Kawai Y, Yamaguchi-Kabata Y, Nagasaki M "TIGAR2: sensitive and accurate estimation of transcript isoform expression with longer RNA-Seq reads." BMC Genomics. 2014;15 Suppl 10:S5. Dec 12. https://doi.org/10.1186/1471-2164-15-S10-S5
PMID: 25560536
PMCID: PMC4304212
Nariai N, Hirose O, Kojima K, Nagasaki M "TIGAR: transcript isoform abundance estimation method with gapped alignment of RNA-Seq data by variational Bayesian inference." Bioinformatics. 2013 Sep 15;29(18):2292-9. https://doi.org/10.1093/bioinformatics/btt381
PMID: 23821651
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