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dropEst

dropEst

dropEst is a pipeline tool to estimate molecular counts in droplet-based single-cell RNA-seq (scRNA-seq) data. The dropEst package correct barcodes, classifies cell quality, computes diagnostic information about the libraries, corrects sequencing errors, and composition bias.

Topic

Gene expression; RNA-Seq

Details

  • Operation: Differential gene expression analysis; RNA-Seq analysis
  • Software interface: Command-line user interface
  • Language: C++, R
  • Operating system: Linux
  • License: GNU General Public License v3
  • Cost: Free
  • Version name: -
  • Credit: National Institutes of Health (NIH), National Science Foundation (NSF), the National Institute of Diabetes and Digestive and Kidney Diseases, part of the United States National Institutes of Health (NIDDK).
  • Contact: Peter V. Kharchenko peter.kharchenko _at_ post.harvard.edu
  • Collection: -

Publications

Petukhov V, Guo J, Baryawno N, Severe N, Scadden DT, Samsonova MG, Kharchenko PV "dropEst: Pipeline for Accurate Estimation of Molecular Counts in Droplet-Based Single-Cell RNA-seq Experiments" Genome Biol. 2018 Jun 19;19(1):78. https://doi.org/10.1186/s13059-018-1449-6
PMID: 29921301
PMCID: PMC6010209


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