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easyRNASeq

easyRNASeq

easyRNASeq is a tool to quantify RNA-seq expression data. The package also has functions for retrieving annotations, read count summarization by feature. It reports Reads per kilobase per million mapped reads (RPKM).

Topic

RNA-Seq; Sequence analysis

Details

  • Operation: Gene expression profiling
  • Input: SAM, BAM
  • Output: GFF, GFF3
  • Software interface: Command-line user interface
  • Language: R
  • Operating system: Linux; Mac OS X; Microsoft Windows
  • License: Artistic-2.0
  • Cost: Free
  • Version name: 2.22.2
  • Maturity: Mature
  • Credit: European Molecular Biology Laboratory (EMBL), ERASysBio, the National Institutes of Health (NIH).
  • Contact: Nicolas Delhomme nicolas.delhomme _at_ umu.se
  • Collection: Bioconductor

Publications

Delhomme N, Padioleau I, Furlong EE, Steinmetz LM "easyRNASeq: a bioconductor package for processing RNA-Seq data." Bioinformatics. 2012 Oct 1;28(19):2532-3. Epub 2012 Jul 30. https://doi.org/10.1093/bioinformatics/bts477
PMID: 22847932
PMCID: PMC3463124


Huber W, Carey VJ, Gentleman R, Anders S, Carlson M, Carvalho BS, Bravo HC, Davis S, Gatto L, Girke T, Gottardo R, Hahne F, Hansen KD, Irizarry RA, Lawrence M, Love MI, MacDonald J1, Obenchain V, Oleś AK, Pagès H, Reyes A, Shannon P, Smyth GK, Tenenbaum D, Waldron L, Morgan M "Orchestrating high-throughput genomic analysis with Bioconductor." Nat Methods. 2015 Feb;12(2):115-21. https://doi.org/10.1038/nmeth.3252
PMID: 25633503
PMCID: PMC4509590


Gentleman RC, Carey VJ, Bates DM, Bolstad B, Dettling M, Dudoit S, Ellis B, Gautier L, Ge Y, Gentry J, Hornik K, Hothorn T, Huber W, Iacus S, Irizarry R, Leisch F, Li C, Maechler M, Rossini AJ, Sawitzki G, Smith C, Smyth G, Tierney L, Yang JY, Zhang J. "Bioconductor: open software development for computational biology and bioinformatics." Genome Biol. 2004;5(10):R80. Epub 2004 Sep 15. https://doi.org/10.1186/gb-2004-5-10-r80
PMID: 15461798
PMCID: PMC545600


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