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BioNanoAnalyst is a tool for evaluating potential mis-assemblies in reference genomes using optical maps. It is a cross-platform graphical user interface (GUI) program. It produces GFF3 output of potential mis-assembled regions and it also has a zoom in visualization of such genomic locations.


Sequence assembly


  • Operation: Sequence assembly validation
  • Input: BioNano optical map
  • Output: GFF3
  • Software interface: Graphical user interface
  • Language: Python
  • Operating system: Linux; Mac OS X; Microsoft Windows
  • License: GPLv3
  • Cost: Free
  • Version name: -
  • Credit: Australian Research Council
  • Contact: yuxuan.yuan _at_
  • Collection: -


Yuan Y, Bayer PE, Scheben A, Chan CK, Edwards D "BioNanoAnalyst: a visualisation tool to assess genome assembly quality using BioNano data." BMC Bioinformatics. 2017 Jun 30;18(1):323.
PMID: 28666410
PMCID: PMC5493081

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