For better experience, turn on JavaScript!



Phusion is a tool for de novo genome assembly. The assembler is modular in its design and uses the concept of mathematically defined repeats to find repeats and then uses another program, PHRAP as its assembly algorithm. The clustering of reads in pre-assembly step allows parallelization in the assembly stage.


Sequence assembly


  • Operation: Genome assembly
  • Software interface: Command-line user interface
  • Language: -
  • Operating system: Linux
  • License: GPLv3
  • Cost: Free
  • Version name: -
  • Credit: The Sanger Institute; Genome Sequencing Center, Washington University School of Medicine
  • Contact: jcm _at_
  • Collection: -


Mullikin JC, Ning Z "The phusion assembler." Genome Res. 2003 Jan;13(1):81-90.
PMID: 12529309
PMCID: PMC430959

Download and documentation

If you find errors, please report here.