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BraCeR

BraCeR

An R tool to reconstruct full-length B-cell-receptor (BCR) sequences from paired-end or single-end single-cell RNA-seq data. BraCeR is an extension of the TraCeR pipeline and can also determine clonality, estimate somatic hypermutations (SHMs), isotype switching, and create resource files for analyses of additional species. The default version is human and mice.

Topic

Cell biology; RNA-seq; Immunoproteins, genes and antigens

Details

  • Operation: Peptide immunogenicity prediction
  • Software interface: Command-line user interface; Library
  • Language: Python/R
  • Operating system: Microsoft Windows, Mac OS X, Linux
  • License: Apache-2.0
  • Cost: Free
  • Version name: 1.11
  • Maturity: Stable
  • Credit: University of Oslo world-leading research program on human immunology, South-Eastern Norway Regional Health Authority, Stiftelsen KG Jebsen, he Wellcome Trust, ERC.
  • Contact: Michael J. T. Stubbington mjt.stubbington _at_ gmail.com
  • Collection: -

Publications

Lindeman I, Emerton G, Mamanova L, Snir O, Polanski K, Qiao SW, Sollid LM, Teichmann SA, Stubbington MJT "BraCeR: B-cell-receptor reconstruction and clonality inference from single-cell RNA-seq." Nat Methods. 2018 Aug;15(8):563-565. https://doi.org/10.1038/s41592-018-0082-3
PMID: 30065371


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