CHOPCHOP is a web-based tool to select target sites for CRISPR/Cas9- or TALEN-directed mutagenesis. The CHOPCHOP algorithm predicts off-target binding of single-guide RNAs (sgRNAs) and TALENs using effective sequence alignment methods and visualizes the candidate target sites, restriction sites, primer candidates, together with color-coded quality scores.
Sequence analysis
Montague TG, Cruz JM, Gagnon JA, Church GM, Valen E "CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing." Nucleic Acids Res. 2014; 42(Web Server issue):W401-7 https://doi.org/10.1093/nar/gku410
PMID: 24861617
PMCID: PMC4086086
Labun K, Montague TG, Gagnon JA, Thyme SB, Valen E "CHOPCHOP v2: a web tool for the next generation of CRISPR genome engineering." Nucleic Acids Res, 2016 https://doi.org/10.1093/nar/gkw398
PMID: 27185894
PMCID: PMC4987937
Labun K, Montague TG, Krause M, Torres Cleuren YN, Tjeldnes H, Valen E "CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing." Nucleic Acids Res, 2019 https://doi.org/10.1093/nar/gkz365
PMID: 31106371
PMCID: PMC6602426
If you find errors, please report here.
SECTIONS
TutorialsFind thousands of Bioinformatics and Life Science software tools and databases in the newly launched
Ads