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A software package for the analysis of DNA polymorphisms using data from a multiple sequence aligned data. Features: Estimate various measures of DNA sequence variation within and between populations. Estimate linkage disequilibrium, recombination, gene flow and gene conversion parameters. A number of neutrality tests such as, the Hudson, Kreitman and Aguadé (1987), Tajima (1989), McDonald and Kreitman (1991), Fu and Li (1993), and Fu (1997), Ramos-Onsins and Rozas (2002), Achaz (2009) tests, and compute confidence intervals by the coalescent. Input data formats: FASTA; MEGA; NBRF/PIR; NEXUS; PHYLIP; HapMap3; VCF; Multi-MSA; Arlequin.


Biology, Genetics, Molecular genetics, Genetic variation, DNA polymorphism


  • Operation: Analysis, Sequence analysis, Nucleic acid sequence analysis, Genetic variation analysis
  • Software interface: Desktop graphical user interface
  • Language: Visual Basic.NET
  • Operating system: Microsoft Windows
  • License: Not stated
  • Cost: Free for non-commercial
  • Version name: 6.12
  • Credit: Rozas J, Ferrer-Mata A, Sánchez-DelBarrio JC, Guirao-Rico S, Librado
  • Contact: jrozas at
  • Collection: -


Rozas J, Ferrer-Mata A, Sánchez-DelBarrio JC, Guirao-Rico S, Librado P, Ramos-Onsins SE, Sánchez-Gracia A "DnaSP 6: DNA Sequence Polymorphism Analysis of Large Data Sets." Mol. Biol. Evol. 2017; 34(12):3299-3302
PMID: 29029172

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