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A web-based tool to predict the effect of amino acid mutations in protein sequences by pair-wise alignments of protein sequences in the UniProt Swiss-Prot/TrEMBL database and estimates deleteriousness utilizing a taxonomic distance of the species.


Biology, Genetics, DNA polymorphism


  • Operation: Prediction and recognition
  • Software interface: Web user interface
  • Language: -
  • Operating system: Linux, Mac OS X, Microsoft Windows
  • License: Apache License v2
  • Cost: Free for academic not for profit research only.
  • Version name: 2.0
  • Credit: Matthew Jacobson, Paul Pavlidis, Erich Kuechler, Eric T.C. This work was funded by Canadian Institutes of Health Research (CIHR); Natural Sciences and Engineering Research Council of Canada (NSERC); Genome Canada and Genome BC [175REG to J.G.]; Michael Smith Foundation for Health Research (MSFHR) [CI-SCH-03020(11-1) to J.G.]. Funding for open access charge: CIHR; NSERC; Genome Canada and Genome BC [175REG to J.G.]; MSFHR [CI-SCH-03020(11-1) to J.G.].
  • Contact: nmalhis at, gsponer at
  • Collection: -


Malhis N, Jones SJM, Gsponer J "Improved measures for evolutionary conservation that exploit taxonomy distances." Nat Commun 2019; 10(1):1556
PMID: 30952844
PMCID: PMC6450959

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