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A web tool to predict the effect of single nucleotide polymorphism (SNP) on protein function.


Biology, Genetics, DNA polymorphism


  • Operation: Prediction and recognition
  • Software interface: Command-line interface, web user interface
  • Language: Perl
  • Operating system: Linux, Mac OS X
  • License: Freely available to the academic community. Commercial licensees should contact Steven Henikoff.
  • Cost: -
  • Version name: -
  • Credit: Tai Pang Yong, Jorja Henikoff, Lakshmi Radhakrishnan, Anthony Youzhi Cheng, and Qiangze Hoi.
  • Contact:
  • Collection: -


Ngak-Leng Sim Prateek Kumar Jing Hu Steven Henikoff Georg Schneider Pauline C Ng. "SIFT web server: predicting effects of amino acid substitutions on proteins." Nucleic Acids Research, Volume 40, Issue W1, July 2012, Pages W452–W457.
PMID: 22689647
PMCID: PMC3394338

Prateek Kumar, Steven Henikoff, Pauline C Ng. "Predicting the effects of coding non-synonymous." Nature Protocols 4, - 1073 - 1081.
PMID: 19561590

Robert Vaser, Swarnaseetha Adusumalli, Sim Ngak Leng, Mile Sikic, Pauline C Ng. "SIFT missense predictions for genomes." Nature Protocols volume 11, pages 1–9.
PMID: 26633127

Ng PC, Henikoff S. "SIFT: Predicting amino acid changes that affect protein function." Nucleic Acids Res. 2003 Jul 1;31(13):3812-4.
PMID: 12824425
PMCID: PMC168916

Ng PC, Henikoff S. "Predicting deleterious amino acid substitutions." Genome Res. 2001 May;11(5):863-74.
PMID: 11337480
PMCID: PMC311071

Ng PC, Henikoff S. "Accounting for human polymorphisms predicted to affect protein function." Genome Res. 2002 Mar;12(3):436-46.
PMID: 11875032
PMCID: PMC155281

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