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A tool specifically designed for combinatorial protein engineering. ANTICALIgN can construct multiple sequence alignments (MSAs) based on a template reference sequence and global sequence alignments. Available from the Authors upon request.


Protein structure analysis; Sequence analysis


  • Operation: Multiple sequence alignment; protein analysis
  • Input: FASTA; GCG; Plain text; ApE; .seq; ClustalW
  • Output: ANTICALIgN; FASTA; GCG; Newick; Phylip; PNG; ClustalW
  • Software interface: Graphical user interface
  • Language: Java
  • Operating system: Linux; MAC OS X; Microsoft Windows
  • License: Not stated
  • Cost: Free
  • Version name: -
  • Credit: Stefan Achatz, Technische Universit√§t M√ľnchen
  • Contact: Arne Skerra skerra _at_
  • Collection: -


Jarasch A, Kopp M, Eggenstein E, Richter A, Gebauer M, Skerra A "ANTICALIgN: visualizing, editing and analyzing combined nucleotide and amino acid sequence alignments for combinatorial protein engineering." Protein Eng Des Sel. 2016 Jul;29(7):263-70.
PMID: 27261456

Download and documentation

  • Home page:

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