PnpProbs is a multiple sequence alignment (MSA) tool. It operates by assigning sequences into two, distantly and "normally" related, groups and uses a guide tree solely for "normally" related sequences. For distantly related sequences, it applies a non-progressive approach to generate a multiple sequence alignment (MSA).
Ye Y, Lam TW, Ting HF "PnpProbs: a better multiple sequence alignment tool by better handling of guide trees." BMC Bioinformatics. 2016 Aug 31;17 Suppl 8:285. https://doi.org/10.1186/s12859-016-1121-7
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