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HISAT2

HISAT2

A tool to map DNA and RNA sequences to one or more genomes. The HISAT2 algorithm uses an extension of the Burrows-Wheeler transform (BWT) to generate graphs, a new graph FM index (GFM), and a Hierarchical Graph FM index (HGFM) to index a whole-genome and population of genomes.

Topic

RNA-seq; Sequencing; Mapping

Details

  • Operation: Sequence alignment; Mapping
  • Software interface: Command-line user interface
  • Language: Python
  • Operating system: Linux; Mac OS X; Microsoft Windows
  • License: GNU General Public License v3
  • Cost: Free
  • Version name: 2.1.0
  • Maturity: Mature
  • Credit: he National Human Genome Research Institute (US National Institutes of Health)
  • Contact: Ben Langmead langmea _at_ cs.jhu.edu | Daehwan Kim infphilo _at_ gmail.com | Steven L Salzberg salzberg _at_ jhu.edu
  • Collection: -

Publications

Kim D, Langmead B, Salzberg SL "HISAT: a fast spliced aligner with low memory requirements." Nat Methods. 2015 Apr;12(4):357-60. https://doi.org/10.1038/nmeth.3317
PMID: 25751142
PMCID: PMC4655817


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