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PrimerSeq

PrimerSeq

PrimerSeq is a tool to design RT-PCR primers for the evaluation of alternative splicing using RNA-seq data.

Topic

Probes and primers; RNA; RNA-seq; RNA-seq; RNA splicing

Details

  • Operation: PCR primer design
  • Software interface: Graphical user interface
  • Language: Python;Java
  • Operating system: Mac OS X; Microsoft Windows
  • License: Apache-2.0;Boost-1.0;GNU GPL v2.0, v3.0, v2.1; The MIT Licence
  • Cost: Free with limitations
  • Version name: 1.1
  • Maturity: Mature
  • Credit: -
  • Contact: primerseq _at_ gmail.com
  • Collection: -

Publications

Tokheim C, Park JW, Xing Y "PrimerSeq: Design and visualization of RT-PCR primers for alternative splicing using RNA-seq data." Genomics Proteomics Bioinformatics. 2014 Apr;12(2):105-9. 10.1016/j.gpb.2014.04.001. Epub 2014 Apr 18. https://doi.org/10.1016/j.gpb.2014.04.001
PMID: 24747190
PMCID: PMC4411361


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