Scripture is a tool for de novo assembly of RNA-seq full-length gene transcriptome data. The Scripture algorithm needs both reads and a genome sequence, and can handle strandedness.
RNA-Seq; Transcriptomics; Gene expression
Guttman M, Garber M, Levin JZ, Donaghey J, Robinson J, Adiconis X, Fan L, Koziol MJ, Gnirke A, Nusbaum C, Rinn JL, Lander ES, Regev A "Ab initio reconstruction of cell type-specific transcriptomes in mouse reveals the conserved multi-exonic structure of lincRNAs." Nat Biotechnol. 2010 May;28(5):503-10. https://doi.org/10.1038/nbt.1633
PMID: 20436462
PMCID: PMC2868100
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