Sequgio is a tool to estimate the expression of isoforms and their distribution in RNA-seq datasets. The Sequgio algorithm uses a joint statistical model to assess nonuniformly distributed reads. However, the Authors no longer maintain the package.
RNA-seq
Suo C, Calza S, Salim A, Pawitan Y "Joint estimation of isoform expression and isoform-specific read distribution using multisample RNA-Seq data." Bioinformatics. 2014 Feb 15;30(4):506-13. Epub 2013 Dec 3. https://doi.org/10.1093/bioinformatics/btt704
PMID: 24307704
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