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StringTie

StringTie

A tool to assemble RNA-seq sequence alignments into transcripts. The StringTie algorithm can optionally make de novo assemblies and uses a novel network flow algorithm.

Topic

Transcriptomics; RNA-seq

Details

  • Operation: Transcriptome assembly; RNA-Seq analysis
  • Input: BAM
  • Output: -
  • Software interface: Command-line user interface
  • Language: C++;C
  • Operating system: Linux; Mac OS X
  • License: The MIT License
  • Cost: Free
  • Version name: -
  • Maturity: Stable
  • Credit: The US National Institutes of Health (NIH), the Cancer Prevention and Research Institute of Texas
  • Contact: mpertea _at_ jhu.edu
  • Collection: -

Publications

Pertea M, Pertea GM, Antonescu CM, Chang TC, Mendell JT, Salzberg SL "StringTie enables improved reconstruction of a transcriptome from RNA-seq reads." Nat Biotechnol. 2015 Mar;33(3):290-5. https://doi.org/10.1038/nbt.3122
PMID: 25690850
PMCID: PMC4643835


Pertea M, Kim D, Pertea GM, Leek JT, Salzberg SL "Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown." Nat Protoc. 2016 Sep;11(9):1650-67. https://doi.org/10.1038/nprot.2016.095
PMID: 27560171
PMCID: PMC5032908


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