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zUMIs

zUMIs

A pipeline tool for analysis of RNA-seq data comprising sample-specific barcodes and unique molecular identifiers. The zUMIs algorithm can identify cells by a read distribution. Besides, it has a function to downsample varying library sizes.

Topic

RNA-seq; Mapping; Sequence analysis

Details

  • Operation: Mapping; Filtering; RNA-Seq analysis
  • Software interface: Command-line user interface
  • Language: R;Perl
  • Operating system: Linux
  • License: GNU General Public License v3
  • Cost: Free
  • Version name: 2.5.6
  • Maturity: Mature
  • Credit: Deutsche Forschungsgemeinschaft (DFG)
  • Contact: hellmann _at_ bio.lmu.de;sparekh _at_ age.mpg.de
  • Collection: -

Publications

Parekh S, Ziegenhain C, Vieth B, Enard W, Hellmann I "zUMIs - A fast and flexible pipeline to process RNA sequencing data with UMIs." Gigascience. 2018 Jun 1;7(6). pii: 5005022. https://doi.org/10.1093/gigascience/giy059
PMID: 29846586
PMCID: PMC6007394


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