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BUSCO is a tool to assess completeness of genome assembly, gene set and transcriptome. It is based on the concept of single-copy orthologs that should be highly conserved among the closely related species. For example, users who wish to study the completeness of a mammalian genome assembly will use single-copy orthologs discovered among mammalian species.


Sequence assembly assessment


  • Operation: Genome assembly assessment; Gene set assessment; Transcriptome assessment
  • Input: FASTA
  • Output: -
  • Software interface: Command-line user interface
  • Language: Python
  • Operating system: Linux
  • License: The MIT licence
  • Cost: Free
  • Version name: -
  • Credit: Swiss Institute of Bioinformatics
  • Contact: evgeny.zdobnov _at_
  • Collection: -


Simao FA, Waterhouse RM, Ioannidis P, Kriventseva EV, Zdobnov EM "BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs." Bioinformatics. 2015 Oct 1;31(19):3210-2. Epub 2015 Jun 9.
PMID: 26059717

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